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Monoclonal antibodies against human BAP31 for immunocytochemistry
Chaojun Song1, Fuli Wang, Zhuwei Xu
1Department of Immunology, The Fourth Military Medical University, Xi'an, P.R. China. scj0530@yahoo.com.cn
Abstract:
Human BAP31 is a 28 kDa polytopic integral protein of the ER and part of a large BAP hetero-oligomeric complex that includes the related BAP29 protein and connections to actomyosin. BAP31 interacts with mIgD, cellubrevin, major histocompatibility complex class I, and BCL-2/BCL-X(L), and plays an important role in regulating the egress of these proteins and in apoptosis. Northern blot analyses have revealed BAP31 RNA transcripts in many tissues, including thymus, spleen, brain, kidney, testis, liver, and lung. However, prominent BAP31 protein expression analyzed by immunohistochemistry is restricted to a minority of cells in normal human tissue. Further studies should be made to verify the expression profiles of BAP31 in the protein level. Production of high affinity MAbs suitable for immunohistochemical staining has lagged. Here we generate a set of MAbs that could be used in Western blot, immunoprecipitation, and immunocytochemistry, providing a new powerful tool for investigation of expression profile of BAP31 protein and furthers the study of BAP31 functions.
Insights
Researchers developed new monoclonal antibodies (MAbs) to study human BAP31 protein expression. These tools aid in understanding BAP31
Area of Science:
- Molecular Biology
- Cell Biology
- Immunology
Background:
- Human BAP31 is an ER-localized protein involved in protein transport and apoptosis.
- BAP31 protein expression is reportedly limited in normal human tissues, despite widespread RNA transcripts.
- A lack of high-affinity monoclonal antibodies (MAbs) has hindered detailed protein expression studies.
Purpose of the Study:
- To generate novel, high-affinity MAbs for BAP31.
- To provide tools for investigating BAP31 protein expression profiles.
- To facilitate further research into BAP31 functions.
Main Methods:
- Generation of a set of monoclonal antibodies (MAbs).
- Validation of MAbs for Western blot, immunoprecipitation, and immunocytochemistry.
Main Results:
- Successfully generated MAbs suitable for various immunological assays.
- These MAbs offer a powerful new tool for BAP31 research.
Conclusions:
- The developed MAbs enable precise investigation of BAP31 protein expression.
- These reagents will advance the study of BAP31's role in cellular processes and disease.
