Related Experiment Video
Updated: Jun 22, 2026

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry
Published on: September 13, 2014
Methods for calculating the entropy and free energy and their application to problems involving protein flexibility
Hagai Meirovitch1, Srinath Cheluvaraja, Ronald P White
1Department of Computational Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, USA. hagaim@pitt.edu
Abstract:
The Helmholtz free energy, F and the entropy, S are related thermodynamic quantities with a special importance in structural biology. We describe the difficulties in calculating these quantities and review recent methodological developments. Because protein flexibility is essential for function and ligand binding, we discuss the related problems involved in the definition, simulation, and free energy calculation of microstates (such as the alpha-helical region of a peptide). While the review is broad, a special emphasize is given to methods for calculating the absolute F (S), where our HSMC(D) method is described in some detail.
More Related Videos
08:13Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
Published on: March 4, 2017
09:15Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions
Published on: November 21, 2017
Related Concept Videos
Gibbs Free Energy
The Equilibrium Binding Constant and Binding Strength
The Equilibrium Binding Constant and Binding Strength
An Introduction to Free Energy
Effects of Temperature on Free Energy
Calculating Standard Free Energy Changes