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Related Concept Videos

MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
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Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
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Assembly of Signaling Complexes

Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
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Microtubule Associated Proteins (MAPs)

Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...

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Mapping Dysfunctional Protein-Protein Interactions in Disease
09:39

Mapping Dysfunctional Protein-Protein Interactions in Disease

Published on: October 24, 2025

Sequence patches on MAPK surfaces define protein-protein interactions.

Gary L Johnson1, Shawn M Gomez

  • 1Department of Pharmacology and Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599-7365, USA. glj@med.unc.edu

Genome Biology
|June 13, 2009
PubMed
Summary

Redesigning protein surface patches on mitogen-activated protein kinases can alter cell signaling network topology. This research explores protein modularity and its impact on biological pathways.

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07:44

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In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces

Published on: March 19, 2010

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Systems Biology

Background:

  • Mitogen-activated protein kinases (MAPKs) are crucial in cellular signal transduction.
  • Protein modularity allows for functional diversification and network rewiring.

Purpose of the Study:

  • To investigate how modifications to specific protein regions affect signaling network structure.
  • To understand the role of protein surface patches in MAPK signaling network topology.

Main Methods:

  • Computational modeling of protein-protein interactions.
  • Analysis of signaling network dynamics.
  • In silico redesign of protein surface patches.

Main Results:

  • Altering surface patches on MAPKs demonstrably changed the overall signaling network topology.
  • Specific modifications led to predictable alterations in signal flow and network behavior.

Conclusions:

  • Protein surface patches are key determinants of MAPK signaling network topology.
  • Targeted protein redesign offers a strategy to modulate cellular signaling pathways.