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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jun 22, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Protein microarrays.

Joseph Fasolo¹, Michael Snyder

¹Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA.

Methods in Molecular Biology (Clifton, N.J.)

|June 13, 2009

Summary

This summary is machine-generated.

Researchers created yeast protein microarrays for broad screening. These powerful tools enable unbiased analysis of protein interactions and functions, aiding in drug discovery and understanding cellular regulation.

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Published on: August 23, 2012

Area of Science:

Proteomics
Biochemistry
Molecular Biology

Background:

Protein microarrays offer a platform for high-throughput proteome analysis.
Previous methods lacked comprehensive coverage of the entire proteome.

Purpose of the Study:

To construct protein microarrays representing nearly the entire yeast proteome.
To demonstrate the utility of these arrays for diverse biochemical screening applications.

Main Methods:

Overexpression and high-throughput purification of yeast proteins.
Printing purified proteins onto microscope slides to create microarrays.

Main Results:

Construction of protein microarrays covering a significant portion of the yeast proteome.
Demonstrated utility in screening for protein-protein interactions, lipid/nucleic acid binding, and small molecule targets.

Conclusions:

Yeast protein microarrays are powerful tools for unbiased, large-scale proteomic studies.
These arrays facilitate systematic characterization of protein activities, interactions, and regulation.