Analysis of protein-protein interactions using array-based yeast two-hybrid screens

Seesandra V Rajagopala1, Peter Uetz

  • 1J. Craig Venter Institute, Rockville, MD 20850, USA.

Summary

Array-based yeast two-hybrid (Y2H) methods offer comprehensive protein-protein interaction analysis using defined libraries. While resource-intensive for large genomes, they minimize false positives for robust interactome studies.