Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Transgenic Organisms00:53

Transgenic Organisms

Overview
Transgenic Organisms00:53

Transgenic Organisms

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Network-pharmacology-guided elucidation of Cornus officinalis-mediated protection against paraquat-induced neurotoxicity via restoration of dopaminergic and GABAergic signaling pathways.

Pesticide biochemistry and physiology·2026
Same author

Financial Toxicity in Cervical Cancer Patients Undergoing Radical Treatment With External Beam Radiation and Brachytherapy at a Tertiary New Zealand Radiation Oncology Centre.

Journal of medical imaging and radiation oncology·2026
Same author

A Metal-Based Heterojunction for Controlled Release of Multiple Cations and Reactive Oxygen Species Inhibiting Multidrug-Resistant Bacteria <i>In Vitro</i> and <i>In Vivo</i>.

ACS applied materials & interfaces·2025
Same author

Therapeutic Effects of <i>Hemerocallis citrina</i> Baroni Extract on Animal Models of Neurodegenerative Diseases Through Serotonin and HLH-30/TFEB-Dependent Mechanisms.

International journal of molecular sciences·2025
Same author

Chemical characterization and antioxidant potential of Arthrospira sp., Thalassiosira sp., and Raphidonema sp.

Food chemistry·2024
Same author

Profession-based manual therapy nomenclature: exploring history, limitations, and opportunities.

The Journal of manual & manipulative therapy·2023
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Jun 22, 2026

Hyperactive piggyBac Transposase-mediated Germline Transformation in the Fall Armyworm, Spodoptera frugiperda
05:20

Hyperactive piggyBac Transposase-mediated Germline Transformation in the Fall Armyworm, Spodoptera frugiperda

Published on: September 23, 2021

Transgenic Hypericum perforatum.

G Franklin1, Margarida M Oliveira, Alberto C P Dias

  • 1Departmento de Biologia, Universidade do Minho, Campus de Gualtar, Braga, Portugal.

Methods in Molecular Biology (Clifton, N.J.)
|June 13, 2009
PubMed
Summary
This summary is machine-generated.

We developed a plant transformation protocol for Hypericum perforatum (St. John's Wort) using particle bombardment. This method enables stable gene expression and integration, advancing functional genomics research in this species.

More Related Videos

Soybean Hairy Root Transformation for the Analysis of Gene Function
07:34

Soybean Hairy Root Transformation for the Analysis of Gene Function

Published on: May 5, 2023

Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat (Fagopyrum tataricum)
08:12

Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat (Fagopyrum tataricum)

Published on: March 11, 2020

Related Experiment Videos

Last Updated: Jun 22, 2026

Hyperactive piggyBac Transposase-mediated Germline Transformation in the Fall Armyworm, Spodoptera frugiperda
05:20

Hyperactive piggyBac Transposase-mediated Germline Transformation in the Fall Armyworm, Spodoptera frugiperda

Published on: September 23, 2021

Soybean Hairy Root Transformation for the Analysis of Gene Function
07:34

Soybean Hairy Root Transformation for the Analysis of Gene Function

Published on: May 5, 2023

Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat (Fagopyrum tataricum)
08:12

Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat (Fagopyrum tataricum)

Published on: March 11, 2020

Area of Science:

  • Plant Biology
  • Functional Genomics
  • Plant Biotechnology

Background:

  • Plant transformation is crucial for crop improvement and understanding gene function.
  • Functional genomics research increasingly relies on plant transformation techniques.
  • A robust transformation system for Hypericum perforatum was lacking.

Purpose of the Study:

  • To establish a reliable plant transformation protocol for Hypericum perforatum.
  • To enable the study of gene function and regulation in H. perforatum.
  • To provide a method for generating transgenic St. John's Wort.

Main Methods:

  • Particle bombardment was used to deliver foreign DNA into Hypericum perforatum organogenic nodule explants.
  • Transgenic shoot regeneration was achieved under selection pressure.
  • Molecular analyses confirmed transgene integration and expression.

Main Results:

  • Successfully established a transformation system for Hypericum perforatum.
  • Demonstrated stable integration of ss-glucuronidase and hygromycin phosphotransferase genes.
  • Generated phenotypically normal transgenic H. perforatum plants.

Conclusions:

  • The described particle bombardment method is effective for Hypericum perforatum transformation.
  • This protocol facilitates functional genomics research in H. perforatum.
  • The developed system allows for the creation of transgenic St. John's Wort with stable gene expression.