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Related Concept Videos

Plasmids01:28

Plasmids

Plasmids are extrachromosomal DNA molecules found in bacteria, archaea, and some eukaryotic microbes like yeast. These small, circular DNA structures typically contain fewer than 30 genes, although some may exist linearly. Plasmids vary in their number within a cell, known as copy number. Single-copy plasmids are present in one copy per cell and multi-copy plasmids are present in multiple copies, reaching over 100 copies per cell.Plasmids usually replicate independently of the chromosomal DNA...
DNA Bacteriophages01:26

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
LTR Retrotransposons03:08

LTR Retrotransposons

LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...

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Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli
06:56

Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli

Published on: March 24, 2023

Plasmid replicon typing.

Timothy J Johnson1, Lisa K Nolan

  • 1Department of Veterinary and Biomedical Sciences, University of Minnesota, 205, Veterinary Science, Saint Paul, MN, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 13, 2009
PubMed
Summary
This summary is machine-generated.

Plasmid classification is crucial for understanding microbial evolution and health. A new PCR-based method offers a faster, large-scale approach to typing plasmids within Enterobacteriaceae.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Plasmid classification aids in studying their roles in health, environment, and microbial evolution.
  • Traditional incompatibility (Inc) typing, while useful, is time-consuming and not ideal for large-scale applications.

Purpose of the Study:

  • To introduce and elaborate on a recently developed PCR-based typing method for plasmids.
  • To streamline the large-scale typing of plasmids, particularly within the Enterobacteriaceae family.

Main Methods:

  • The study focuses on a polymerase chain reaction (PCR)-based method for plasmid replicon typing.
  • This method enables large-scale plasmid classification based on replication mechanisms.

Main Results:

  • The described PCR-based method significantly streamlines plasmid typing.
  • It is believed to be highly applicable for large-scale plasmid studies.

Conclusions:

  • The new PCR-based typing method offers an efficient alternative to traditional methods.
  • This advancement facilitates broader research into plasmid diversity and function.