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Related Concept Videos

Applications of Molecular Taxonomy01:20

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Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
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Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
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Internet-based sequence-typing databases for bacterial molecular epidemiology.

Keith A Jolley1

  • 1Department of Zoology and Peter Medawar Building for Pathogen Research, University of Oxford, South Parks Road, Oxford, UK.

Methods in Molecular Biology (Clifton, N.J.)
|June 13, 2009
PubMed
Summary
This summary is machine-generated.

Internet-based databases are essential for microbial epidemiology, standardizing nomenclature and type definitions. These resources enable data sharing between labs, crucial for multilocus sequence typing (MLST) and antigen sequence analysis.

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Area of Science:

  • Microbiology
  • Bioinformatics
  • Epidemiology

Background:

  • Nucleotide sequence-based typing is increasingly used in microbial epidemiology.
  • This necessitates standardized, accessible data repositories for nomenclature and type definitions.

Purpose of the Study:

  • To describe the utility of common Internet-based databases for microbial typing.
  • To provide guidance on using these databases for multilocus sequence typing (MLST) and antigen sequence analysis.

Main Methods:

  • Explanation of allele sequence and profile identification for MLST.
  • Overview of searching and matching isolate records in MLST databases.
  • Description of BLAST searching for antigen sequence databases.

Main Results:

  • Demonstration of how curated databases facilitate data sharing and comparison.
  • Highlighting the importance of these databases in the absence of physical reference samples.

Conclusions:

  • Internet-based databases are critical infrastructure for modern microbial epidemiology.
  • Standardized database use enhances global collaboration and data integrity in microbial typing.