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Updated: Jun 22, 2026

A Fluorescence-based Protocol for Preliminary Screening of Protein Synthesis Inhibitors from Natural Sources
Published on: January 27, 2026
Rapid synthesis, screening, and identification of xanthone- and xanthene-based fluorophores using click chemistry
Junqi Li1, Mingyu Hu, Shao Q Yao
1Department of Chemistry, NUS MedChem Program of the Life Sciences Institute, National University of Singapore, Singapore 117543.
Abstract:
A panel of new fluorophores with emission wavelengths from blue to yellow regions using the Cu(I)-catalyzed 1,3-dipolar cycloaddition reaction of alkyne-functionalized xanthones and xanthenes with various azides have been synthesized. Screening of the "click" products led to the identification of "hit" fluorophores which showed a fluorescence increase upon triazole formation. These novel "click" fluorophores could potentially be used for bioconjugation and bioimaging purposes.

