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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

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Updated: Jun 22, 2026

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
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Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

Towards functional genomics in fish using quantitative proteomics.

Christopher J Martyniuk1, Nancy D Denslow

  • 1Center for Environmental and Human Toxicology, Department of Physiological Sciences, University of Florida, P.O. Box 110885, Gainesville, FL 32611-0885, USA. cmartyni@ufl.edu

General and Comparative Endocrinology
|June 16, 2009
PubMed
Summary
This summary is machine-generated.

Quantitative proteomics offers new insights into fish endocrinology, revealing how androgens impact liver proteins. This approach, using isobaric tagging (iTRAQ), enhances understanding of molecular responses in aquatic toxicology.

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Area of Science:

  • Fish endocrinology
  • Molecular toxicology
  • Proteomics

Background:

  • Gene expression analysis, particularly microarrays, has advanced understanding of fish physiology and toxicology, focusing on steroid nuclear receptor signaling.
  • Novel proteomic tools remain underutilized in fish endocrinology despite their potential.
  • Quantitative proteomic methods, including gel-based and non-gel-based approaches, offer advanced analytical capabilities.

Purpose of the Study:

  • To review quantitative proteomic approaches applicable to fish endocrinology.
  • To demonstrate the utility of isobaric tagging (iTRAQ) for studying proteome changes in fish.
  • To investigate the effects of the androgen 17beta-trenbolone on the fathead minnow liver proteome.

Main Methods:

  • Summary of quantitative proteomic techniques (2D gel electrophoresis, DIGE, SILAC, ICAT, iTRAQ, spectral counting, absolute quantitation).
  • Application of isobaric tagging (iTRAQ) for quantitative proteome analysis.
  • Analysis of liver proteome changes in fathead minnows exposed to 17beta-trenbolone.

Main Results:

  • iTRAQ successfully identified changes in the fathead minnow liver proteome in response to 17beta-trenbolone exposure.
  • The study highlights the potential of quantitative proteomics to reveal molecular mechanisms in fish toxicology.
  • Demonstrated the feasibility of applying advanced proteomic techniques to fish models.

Conclusions:

  • Quantitative proteomics, exemplified by iTRAQ, is a powerful tool for fish endocrinology and toxicology research.
  • Integrating genomic and proteomic data is crucial for understanding complex temporal and regulatory effects.
  • Further application of these novel proteomic tools is encouraged for deeper insights into fish molecular responses.