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Implication of surfactant apoprotein in otitis media with effusion
N Yamanaka1, K Kobayashi, A Kataura
1Department of Otolaryngology, Sapporo Medical College, Japan.
Abstract:
A two-site simultaneous immunoassay using monoclonal antibodies against human surfactant apoprotein (SAP) was used to measure SAP in middle ear effusions (MEEs). In 130 MEE samples from children with otitis media with effusion, SAP was detected in 54 samples (SAP-positive cases, 41.5%). In the remainder, the SAP concentration was below the sensitivity of the immunoassay (SAP-negative cases, 58.5%). A significant difference in periods of observation was found between the SAP-positive cases (17.3 +/- 16.8 months) and the SAP-negative cases (26.2 +/- 22.5 months) (p less than .01). The percentage of positive cases was highest in the serous MEE group (81.2%) and decreased in the purulent MEE group (57%), the mucoid MEE group (30%), and the hyperviscous MEE group (13.6%), in that order. In the purulent MEE group and the mucoid MEE group, the period of observation was significantly shorter in the SAP-positive cases (18.3 +/- 20.4 months and 20.2 +/- 19.4 months) than in the SAP-negative cases (35.9 +/- 24.5 months and 25.4 +/- 18.7 months) (p less than .05). These results suggest that SAP is present in the middle ear cleft and may be a good prognostic predictor of otitis media with effusion in children.
Insights
Human surfactant apoprotein (SAP) was detected in middle ear effusions of children with otitis media. SAP presence may indicate a shorter duration of effusion, suggesting its potential as a prognostic marker.
Area of Science:
- Otolaryngology
- Biochemistry
- Pediatrics
Background:
- Otitis media with effusion (OME) is a common pediatric condition.
- Surfactant apoprotein (SAP) plays a role in pulmonary function and may be present in middle ear effusions (MEEs).
- Understanding biomarkers for OME prognosis is crucial for effective management.
Purpose of the Study:
- To measure SAP levels in MEEs of children with OME.
- To investigate the correlation between SAP presence and clinical characteristics of OME.
- To evaluate SAP as a potential prognostic indicator for OME.
Main Methods:
- A two-site simultaneous immunoassay utilizing monoclonal antibodies against human SAP was employed.
- 130 MEE samples from children diagnosed with OME were analyzed.
- SAP concentrations were quantified, and cases were categorized as SAP-positive or SAP-negative based on assay sensitivity.
Main Results:
- SAP was detected in 41.5% of MEE samples (54 out of 130).
- SAP-positive cases showed a significantly shorter observation period (17.3 months) compared to SAP-negative cases (26.2 months) (p < .01).
- SAP detection rates varied by MEE type, being highest in serous (81.2%) and lowest in hyperviscous (13.6%) effusions.
Conclusions:
- Human surfactant apoprotein is present in the middle ear cleft of children with OME.
- SAP detection in MEEs may be associated with a shorter disease duration.
- SAP shows potential as a prognostic biomarker for otitis media with effusion in children.