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In vivo activity of anprocide alone, and in vitro activity in combination with conventional antibiotics against

Robin K Pettit1, Christine A Weber1, Stacey B Lawrence1

  • 1Cancer Research Institute and Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287, USA.

Journal of Medical Microbiology
|June 17, 2009
PubMed

Insights

The experimental steroidal amide anprocide shows promise against drug-resistant Staphylococcus bacteria. It effectively reduced bacterial counts and demonstrated synergistic effects with existing antibiotics in both planktonic and biofilm models.

Area of Science:

  • Microbiology
  • Pharmacology
  • Infectious Diseases

Background:

  • Rising antimicrobial resistance in Staphylococcus aureus necessitates novel therapeutic strategies.
  • Staphylococcus aureus and Staphylococcus epidermidis are frequently implicated in challenging biofilm infections.

Purpose of the Study:

  • To evaluate the efficacy of the steroidal amide anprocide against Staphylococcus species.
  • To investigate the synergistic potential of anprocide in combination with established antibiotics.

Main Methods:

  • Anprocide's efficacy was assessed in a murine model of topical Staphylococcus aureus infection.
  • Checkerboard assays were employed to determine synergistic interactions with antibiotics against planktonic and biofilm-grown bacteria.

Main Results:

  • Anprocide significantly reduced bacterial colony-forming units in a murine S. aureus infection model.
  • Synergistic effects were observed when anprocide was combined with bacitracin, oxacillin, clindamycin, or ceftriaxone against planktonic bacteria.
  • Anprocide also showed synergy with bacitracin or oxacillin against some biofilm-grown S. aureus and S. epidermidis isolates.

Conclusions:

  • Anprocide exhibits significant antimicrobial activity against Staphylococcus aureus in vivo.
  • Anprocide demonstrates synergistic potential with multiple antibiotics, offering a promising avenue for combating drug-resistant and biofilm-associated staphylococcal infections.

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