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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

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Related Experiment Video

Updated: Jun 22, 2026

Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes
08:44

Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes

Published on: July 30, 2020

In vivo imaging flow cytometer.

Ho Lee, Clemens Alt, Costas M Pitsillides

    Optics Express
    |June 17, 2009
    PubMed
    Summary
    This summary is machine-generated.

    We developed a novel in vivo imaging flow cytometer to capture real-time cell images and data directly within live animals. This technology allows for detailed analysis of circulating T cells and their movement in blood vessels.

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    Related Experiment Videos

    Last Updated: Jun 22, 2026

    Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes
    08:44

    Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes

    Published on: July 30, 2020

    Image-based Flow Cytometry Technique to Evaluate Changes in Granulocyte Function In Vitro
    06:13

    Image-based Flow Cytometry Technique to Evaluate Changes in Granulocyte Function In Vitro

    Published on: December 26, 2014

    Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)
    07:19

    Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

    Published on: June 28, 2017

    Area of Science:

    • Biomedical Engineering
    • Cell Biology
    • In Vivo Imaging

    Background:

    • Current methods for analyzing circulating cells often require ex vivo processing, limiting real-time physiological context.
    • In vivo imaging offers a powerful approach to study cellular dynamics within their native environment.

    Purpose of the Study:

    • To introduce and validate a novel in vivo imaging flow cytometer for simultaneous image and quantitative data acquisition of circulating cells in live animals.
    • To demonstrate the instrument's capability in imaging and measuring the speed of circulating T cells in real time within blood vessels.

    Main Methods:

    • Development of an in vivo imaging flow cytometer utilizing confocal detection of fluorescence and a high-sensitivity CCD camera.
    • Cells passing through a focused light slit in a blood vessel trigger image capture, allowing for real-time snapshot imaging.
    • Validation that detected signal peaks predominantly originate from individual cells.

    Main Results:

    • Demonstrated simultaneous acquisition of fluorescence images and quantitative data from circulating cells in vivo.
    • Successfully imaged circulating T cells and measured their real-time speeds within blood vessels.
    • Confirmed that the majority of detected signals correspond to individual cells.

    Conclusions:

    • The developed in vivo imaging flow cytometer provides a powerful new tool for studying cellular dynamics in live animals.
    • This technology has significant potential applications in fundamental biology and clinical diagnostics.
    • Real-time imaging and quantification of circulating cells offer unprecedented insights into physiological and pathological processes.