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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Total Internal Reflection Fluorescence Microscopy01:05

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
Confocal Fluorescence Microscopy01:16

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Related Experiment Video

Updated: Jun 22, 2026

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons
07:39

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

Published on: July 21, 2018

Fluorescence enhancement by surface gratings.

Yu-Ju Hung, Igor I Smolyaninov, Christopher C Davis

    Optics Express
    |June 17, 2009
    PubMed
    Summary
    This summary is machine-generated.

    Using a gold film with a dielectric grating significantly enhances Rhodamine 6G (R6G) fluorescence. This effect, linked to surface-plasmon-polariton excitation, shows promise for advanced sensing applications.

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    Published on: October 13, 2017

    Area of Science:

    • Optics and Photonics
    • Materials Science
    • Surface Science

    Background:

    • Rhodamine 6G (R6G) is a widely used fluorescent dye.
    • Surface-plasmon-polaritons (SPPs) are collective electron oscillations on metal surfaces.
    • Controlling light-matter interactions at surfaces is crucial for optical applications.

    Purpose of the Study:

    • To investigate the strong fluorescence enhancement of Rhodamine 6G (R6G).
    • To explore the role of dielectric gratings on gold films in modulating R6G fluorescence.
    • To understand the underlying mechanism involving surface-plasmon-polaritons (SPPs).

    Main Methods:

    • Fabrication of a gold film substrate with a deposited dielectric grating.
    • Deposition of a Rhodamine 6G (R6G) layer onto the grating-coated gold film.
    • Systematic study of fluorescence enhancement by varying grating periodicity and excitation light angle of incidence.

    Main Results:

    • Observed strong fluorescence enhancement from the R6G layer.
    • Demonstrated dependence of enhancement on grating periodicity and excitation angle.
    • Correlated enhancement with the excitation of surface-plasmon-polaritons (SPPs) on the gold film.

    Conclusions:

    • The dielectric grating on the gold film effectively enhances R6G fluorescence.
    • The enhancement mechanism is attributed to the excitation of surface-plasmon-polaritons (SPPs).
    • This phenomenon holds potential for developing novel sensing technologies.