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Updated: Jun 22, 2026

Measurement of Chitinase Activity in Biological Samples
Published on: August 22, 2019
Activity measurement of chitosanase by an amperometric biosensor
Hirosuke Tatsumi1, Hajime Katano, Tokuji Ikeda
1International Young Researchers Empowerment Center, Shinshu University, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan. tatsumi@shinshu-u.ac.jp
Abstract:
An amperometric biosensor for the detection of glucosamine (GlcN) and chitosan oligosaccharides ((GlcN)n) was introduced for an activity measurement of chitosanase. By using the biosensor, an increase in the anodic current due to the production of GlcN and (GlcN)n by chitosanase was measured in a chitosan solution. The maximum value of the slope of the current increase was proportional to the enzyme concentration up to 1.4 microg mL(-1). The present method had the advantages of being simple and rapid over the conventional Elson-Morgan method.

