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Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

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Related Experiment Video

Updated: Jun 22, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Quantitative phase microscopy with asynchronous digital holography.

Kevin J Chalut, William J Brown, Adam Wax

    Optics Express
    |June 18, 2009
    PubMed
    Summary
    This summary is machine-generated.

    We developed asynchronous digital holography to measure quantitative phase in biological imaging. This technique captures dynamic cell changes in milliseconds, advancing live cell analysis.

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    Last Updated: Jun 22, 2026

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
    10:28

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

    Published on: July 5, 2016

    Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing
    07:38

    Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing

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    Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)
    07:27

    Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)

    Published on: November 1, 2017

    Area of Science:

    • Biomedical Imaging
    • Optical Physics
    • Cell Biology

    Background:

    • Quantitative phase imaging (QPI) is crucial for label-free analysis of biological specimens.
    • Traditional QPI methods often struggle with dynamic samples due to speed limitations.
    • Acquisition speed is a key bottleneck in observing rapid biological processes.

    Purpose of the Study:

    • To introduce and validate a novel QPI method for high-speed imaging of biological materials.
    • To overcome the temporal limitations of existing phase-shifting interferometry techniques.
    • To enable the study of dynamic changes in live biological samples at millisecond timescales.

    Main Methods:

    • Developed asynchronous digital holography (ADH) utilizing acousto-optic modulators.
    • Employed a moving fringe for phase-shifting interferometry with two near-simultaneous interferograms.
    • Applied ADH to image both standard calibration samples and live cell populations.

    Main Results:

    • Successfully demonstrated quantitative phase measurements with ADH.
    • Achieved millisecond-time-scale imaging of dynamic biological samples.
    • Presented high-resolution quantitative phase images of live cells, showcasing dynamic behavior.

    Conclusions:

    • Asynchronous digital holography provides a robust method for high-speed QPI of biological samples.
    • The technique enables label-free observation of rapid cellular dynamics.
    • ADH represents a significant advancement for live cell imaging and biological research.