Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 22, 2026

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

A reliable method for attaching biological molecules to layer-by-layer self-assemblies.

Robert Wilson1, Maryam Mehrabi, Ian A Prior

  • 1Department of Electrical Engineering and Electronics, Liverpool University, UK. R.Wilson@liv.ac.uk

Chemical Communications (Cambridge, England)
|June 18, 2009
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Maximizing treatment response in patients with neuropathic-subtype postural orthostatic tachycardia syndrome.

Frontiers in neurology·2026
Same author

The Reward Positivity Tracks Positive Reward Prediction Errors From Feedback to Cues During Reinforcement Learning.

Psychophysiology·2026
Same author

Gaboxadol increases resting theta and alpha power without affecting evoked responses in fragile X syndrome in a home-based setting.

Journal of neurodevelopmental disorders·2026
Same author

Highly selective and recyclable vanadium‑promoted Mg-Sr-Al oxide catalyst for n‑butanol ethoxylation.

Scientific reports·2026
Same author

A methodological framework for validating a multi-domain physiological sensor for divers using a scalable data fusion platform.

Frontiers in physiology·2026
Same author

Building capacity in imaging data management.

Journal of microscopy·2026

This study introduces a novel method for securely attaching biological molecules, including oligonucleotides and proteins, to layer-by-layer self-assemblies. This technique enhances the stability and functionality of biomolecular constructs.

Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Molecular Biology

Background:

  • Layer-by-layer (LbL) self-assemblies are versatile platforms for constructing functional materials.
  • Stable immobilization of biological molecules onto surfaces is crucial for biosensor and biomaterial applications.
  • Existing methods for attaching biomolecules to LbL assemblies can lack stability or require complex procedures.

Purpose of the Study:

  • To develop a robust and straightforward method for the stable attachment of diverse biological molecules to LbL self-assemblies.
  • To demonstrate the versatility of the method across different biomolecule types, including oligonucleotides, proteins, and haptens.
  • To enhance the stability and integration of biomolecules within LbL structures for advanced applications.

Main Methods:

More Related Videos

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method
07:56

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method

Published on: May 8, 2014

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay
08:24

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

Published on: September 27, 2021

Related Experiment Videos

Last Updated: Jun 22, 2026

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method
07:56

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method

Published on: May 8, 2014

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay
08:24

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

Published on: September 27, 2021

  • Utilizing layer-by-layer self-assembly techniques to build stratified material architectures.
  • Employing specific chemical or physical strategies for covalent or non-covalent attachment of biomolecules.
  • Characterizing the stability and integrity of the attached biomolecules using relevant analytical methods.

Main Results:

  • Successfully achieved stable immobilization of oligonucleotides, proteins, and haptens onto LbL self-assemblies.
  • Demonstrated enhanced resistance of the attached biomolecules to dissociation and degradation compared to conventional methods.
  • Confirmed the retention of biological activity and functionality of the immobilized molecules.

Conclusions:

  • The developed method provides a reliable approach for the stable integration of biomolecules into LbL self-assemblies.
  • This technique opens avenues for creating advanced biosensors, drug delivery systems, and functional biomaterials.
  • The versatility and stability offered by this method represent a significant advancement in biomolecular surface functionalization.