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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jun 22, 2026

Imaging Flow Cytometry to Study Microbial Autoaggregation
05:19

Imaging Flow Cytometry to Study Microbial Autoaggregation

Published on: September 29, 2023

Multi-wavelength microflow cytometer using groove-generated sheath flow.

Joel P Golden1, Jason S Kim, Jeffrey S Erickson

  • 1Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC, USA.

Lab on a Chip
|June 18, 2009
PubMed
Summary
This summary is machine-generated.

A novel microflow cytometer was developed for particle analysis. This system effectively differentiates coded microspheres and detects phycoerythrin antibody complexes, showing promise for microbial assays.

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Characterizing Microbiome Dynamics &#8211; Flow Cytometry Based Workflows from Pure Cultures to Natural Communities
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09:57

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

Published on: July 12, 2018

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Microfluidics

Background:

  • Traditional flow cytometers face limitations in sample handling and optical interrogation.
  • Developing microfluidic devices offers potential for miniaturized and efficient cellular analysis.

Purpose of the Study:

  • To develop and validate a microflow cytometer with comprehensive sample sheathing and multi-wavelength detection.
  • To assess the device's capability in discriminating coded microspheres and detecting specific biomolecular interactions.
  • To compare the performance of the developed microflow cytometer against a commercial system for microbial assays.

Main Methods:

  • A microfluidic chip was designed with chevron grooves for 3D sheathing of the sample core fluid.
  • Four-wavelength optical interrogation was implemented using diode lasers and optical fibers.
  • Detection of scattered and emitted light was performed using photomultiplier tubes (PMTs) and optical filters.
  • Microsphere coding, phycoerythrin detection, and Escherichia coli assays were conducted and compared to a Luminex flow cytometer.

Main Results:

  • The microflow cytometer successfully discriminated microspheres with varying fluorophore concentrations.
  • Detection of phycoerythrin antibody complex on microsphere surfaces was achieved.
  • The developed system demonstrated comparable performance to a commercial Luminex flow cytometer in Escherichia coli assays.

Conclusions:

  • The developed microflow cytometer provides a robust platform for particle analysis with precise fluidic control and multi-wavelength detection.
  • The system's ability to discriminate coded microspheres and detect specific biomarkers highlights its potential for advanced diagnostic and research applications.
  • This microfluidic approach offers a miniaturized and potentially cost-effective alternative to conventional flow cytometry systems.