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Updated: Jun 22, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
Published on: August 6, 2014
Synthesis and properties of a novel molecular beacon containing a benzene-phosphate backbone at its stem moiety
Yoshihito Ueno1, Akihiro Kawamura, Keiji Takasu
1Department of Biomolecular Science, Faculty of Engineering, Gifu University, Japan. uenoy@gifu-u.ac.jp
Abstract:
This paper describes the synthesis and properties of a novel molecular beacon (MB) containing a benzene-phosphate backbone at its stem moiety. The fluorescence intensity of MBs was found to stabilize by the introduction of the benzene-phosphate backbone at its stem moiety. Furthermore, an MB containing the benzene-phosphate backbone was more resistant to DNase I (endonuclease) than an MB comprising natural DNA and 2'-O-methyl-RNA. These results indicate that the MB with the benzene-phosphate backbone is superior as a molecular beacon as compared to the MB composed of natural DNA and 2'-O-methyl-RNA.
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