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Updated: Jun 22, 2026

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
Published on: June 12, 2018
Discovery of functional miRNA-mRNA regulatory modules with computational methods
Bing Liu1, Jiuyong Li, Anna Tsykin
1School of Computer and Information Science, University of South Australia, Mawson Lakes, SA 5095, Australia. Bing.Liu@unisa.edu.au
Abstract:
The identification of miRNAs and their target mRNAs and the construction of their regulatory networks may give new insights into biological procedures. This study proposes a computational method to discover the functional miRNA-mRNA regulatory modules (FMRMs), that is, groups of miRNAs and their target mRNAs that are believed to participate cooperatively in post-transcriptional gene regulation under specific conditions. The proposed method identifies negatively regulated patterns of miRNAs and mRNAs which associate with cancer and normal conditions, respectively, in a prostate cancer data set. GO and the literature also suggest that they may relate with prostate cancer. It can potentially identify the biologically relevant chains of 'miRNA-->target gene --> condition'.
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