Related Experiment Video
Updated: Jun 22, 2026

Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique
Published on: November 11, 2022
Electrostatic interactions during Kv1.2 N-type inactivation: random-walk simulation
Krzysztof Małysiak1, Zbigniew J Grzywna
1Department of Physical Chemistry and Technology of Polymers, Faculty of Chemistry, Silesian University of Technology, Strzody 9, Gliwice, Poland. krzysztof.malysiak@polsl.pl
Abstract:
N-type inactivation of the Kv1.2 voltage-gated potassium channel is a process in which the N-terminal of the protein (its first 20 amino acids) binds to the open-channel surface, extends and occludes its pore. This process has been experimentally studied in both intact and ShBDelta6-46 channels in which the inactivating peptides are supplied in the bath solution. In this work we provide a qualitative description of N-type inactivation by simulating the random walk of charged inactivating peptides in the electrostatic field that originates from the charges present in the channel and in the cellular membrane. Our results give a deeper insight into the previously reported influence of electrostatics on the rate of N-type inactivation of ShBDelta6-46. We also show how the enchaining of the peptides, i.e., considering the intact form of the channel, influences the N-type inactivation with different charges of those peptides.
More Related Videos
05:37Rapid in-silico Battery Electrolyte Electrochemical Reaction Generation using 3T-VASP Multi-Scale Energy Minimization
Published on: August 22, 2025
11:42Reconstitution of a Transmembrane Protein, the Voltage-gated Ion Channel, KvAP, into Giant Unilamellar Vesicles for Microscopy and Patch Clamp Studies
Published on: January 22, 2015
Related Concept Videos
The Electrical Double Layer
Electrostatic Boundary Conditions in Dielectrics
Consider a case where both the mediums across a boundary are two different dielectric materials. Recall that the electric field and electric displacement are proportional and related through the material's permittivity.
The Debye–Hückel Theory of Electrolyte Solutions
Theory of Strong Electrolytes
Intermolecular Forces
Processes at Electrodes