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Updated: Jun 22, 2026

Generation of Mesenchymal Stem Cells from Human Umbilical Cord Tissue and their Differentiation into the Skeletal Muscle Lineage
Published on: August 31, 2022
Differentiation of multipotent mesenchymal precursors and skeletal myoblasts from human embryonic stem cells
Maria Elena Stavropoulos1, Isabella Mengarelli, Tiziano Barberi
1Beckman Research Institute of City of Hope, Duarte, California, USA.
Abstract:
This unit describes a protocol for the derivation of multipotent mesenchymal precursors from human embryonic stem cells (hESCs). hESCs cultured at low density in the presence of a chemically defined serum-free medium are induced to adopt an endomesodermal fate and later a mesenchymal phenotype. FACS sorting for the surface antigen CD73 is used to purify mesenchymal precursors able to differentiate into fat, bone, cartilage, and skeletal muscle cells. Enrichment in mesenchymal precursors with a myogenic potential is achieved via an additional FACS sorting for the embryonic skeletal muscle surface marker N-CAM.
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