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Updated: Jun 22, 2026

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Published on: April 14, 2023
ZAK negatively regulates RhoGDIbeta-induced Rac1-mediated hypertrophic growth and cell migration
Chih-Yang Huang1, Li-Chiu Yang, Kuan-Yu Liu
1Graduate Institute of Chinese Medical Science, China Medical University, Taichung 404, Taiwan. cyhuang@mail.cmu.edu.tw
Abstract:
RhoGDIbeta, a Rho GDP dissociation inhibitor, induced hypertrophic growth and cell migration in a cultured cardiomyoblast cell line, H9c2. We demonstrated that RhoGDIbeta plays a previously undefined role in regulating Rac1 expression through transcription to induce hypertrophic growth and cell migration and that these functions are blocked by the expression of a dominant-negative form of Rac1. We also demonstrated that knockdown of RhoGDIbeta expression by RNA interference blocked RhoGDIbeta-induced Rac1 expression and cell migration. We demonstrated that the co-expression of ZAK and RhoGDIbeta in cells resulted in an inhibition in the activity of ZAK to induce ANF expression. Knockdown of ZAK expression in ZAK-RhoGDIbeta-expressing cells by ZAK-specific RNA interference restored the activities of RhoGDIbeta.
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