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Updated: Jun 22, 2026

Defining Substrate Specificities for Lipase and Phospholipase Candidates
Published on: November 23, 2016
Methods for detection and characterization of lipases: A comprehensive review
Fariha Hasan1, Aamer Ali Shah1, Abdul Hameed1
1Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan.
Abstract:
Microbial lipases are very prominent biocatalysts because of their ability to catalyze a wide variety of reactions in aqueous and non-aqueous media. The chemo-, regio- and enantio-specific behaviour of these enzymes has caused tremendous interest among scientists and industrialists. Lipases from a large number of bacterial, fungal and a few plant and animal sources have been purified to homogeneity. This article presents a critical review of different strategies which have been employed for the detection, purification and characterization of microbial lipases.
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