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Chemiluminescent substrates increase sensitivity of antigen detection in western blots
G S Sandhu1, B W Eckloff, B C Kline
1Dept. of Biochemistry and Molecular Biology, Mayo Clinic and Foundation, Rochester, MN 55905.
Biotechniques
|July 1, 1991
Abstract:
Replacement of colorimetric substrates in Western blots by chemiluminescent reagents enhances the sensitivity of detection by more than an order of magnitude. Protein levels beyond the detection limit of colorimetric substrates can be consistently detected without modifying pre-existing laboratory protocols.