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Laccase-membrane reactors for decolorization of an acid azo dye in aqueous phase: process optimization
Krishna P Katuri1, S Venkata Mohan, S Sridhar
1Department of Microbiology, National University of Ireland, Galway, Ireland. kpkaturi@yahoo.co.in
Water Research
|June 23, 2009
Summary
This study demonstrates that laccase enzyme effectively decolorizes azo dye without mediators. Immobilizing laccase in chitosan membranes enhances enzyme reusability and decolorization efficiency.
Area of Science:
- Biotechnology
- Environmental Science
- Enzyme Engineering
Background:
- Textile industry wastewater contains persistent azo dyes.
- Effective decolorization methods are crucial for environmental protection.
- Laccase enzymes show potential for dye degradation.
Purpose of the Study:
- To evaluate laccase-membrane reactor configurations for azo dye decolorization.
- To optimize process parameters for maximum decolorization efficiency.
- To assess the reusability of immobilized laccase.
Main Methods:
- Purification of laccase from Pleurotus ostreatus.
- Characterization of laccase using SDS-PAGE.
- Testing various laccase-membrane reactor configurations.
- Optimization of pH, enzyme concentration, dye concentration, temperature, and reaction time.
Main Results:
- Purified laccase showed 8.27-fold increased activity and a molecular weight of 63 kDa.
- Optimal conditions for decolorization were pH 6.0, 1.75 U/ml enzyme, 20 mg/L dye, 30°C, and 120 min.
- Laccase immobilized in chitosan membranes demonstrated high reusability and efficiency.
Conclusions:
- Laccase is effective for azo dye decolorization without redox mediators.
- Chitosan membrane encapsulation offers a reusable and efficient system for laccase-based dye treatment.
- Optimized conditions and reactor design enhance decolorization performance.
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