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Related Experiment Videos

Fibronectin dependent macrophage fibrin binding.

S D Blystone1, L K Weston, J E Kaplan

  • 1Department of Physiology and Cell Biology, Albany Medical College, NY 12208.

Blood
|December 1, 1991
PubMed
Summary

The amino terminus of fibronectin enhances fibrin binding to macrophages. This interaction is a fluid-phase event preceding cell attachment and does not require cellular activation.

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Area of Science:

  • Biochemistry
  • Immunology
  • Cell Biology

Background:

  • Plasma fibronectin (FN) is known to enhance fibrin monomer binding to macrophages.
  • The precise mechanism of this interaction remains to be fully elucidated.

Purpose of the Study:

  • To characterize the mechanism by which fibronectin enhances fibrin binding to macrophages.
  • To identify the specific domains of fibronectin responsible for this activity.

Main Methods:

  • Utilized fibronectin fragments (amino-terminal, gelatin-binding, cell-binding domains) to assess their effect on macrophage fibrin binding.
  • Investigated the role of RGD adhesion sequences, macrophage fixation, exogenous fibronectin preincubation, and endogenous fibronectin removal.

Main Results:

  • Fibronectin fragments containing the amino terminus, but not other domains, mimicked the effect of intact fibronectin.
  • The RGD sequence and macrophage surface interactions were not required for fibronectin-mediated fibrin binding.
  • Fibronectin's enhancement of fibrin binding persisted after macrophage fixation and removal of cell-surface fibronectin.

Conclusions:

  • The amino terminus of fibronectin is critical for mediating fibrin attachment to macrophages.
  • This interaction appears to be a fluid-phase event preceding cellular binding and does not necessitate a cellular response.

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