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Updated: Jun 22, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
Published on: December 9, 2013
LED-based multi-wavelength phase imaging interference microscopy
Abstract:
LED-based multi-wavelength phase imaging interference microscopy combines phase-shifting interferometry with multi-wavelength optical phase unwrapping. This technique consists of a Michelson-type interferometer illuminated with a LED. The reference mirror is dithered for obtaining interference images at four phase quadratures, which are then combined to calculate the phase of the object surface. The 2pi ambiguities are removed by repeating the experiment using two or more LEDs at different wavelengths, which yields phase images of effective wavelength much longer than the original. The resulting image is a profile of the object surface with a height resolution of several nanometers and range of several microns. The interferographic images using broadband sources are significantly less affected by coherent noise.
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