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Updated: Jun 22, 2026

Fluorescence Lifetime Macro Imager for Biomedical Applications

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

Phosphorescence lifetime based oxygen micro-sensing using a digital micromirror device.

Shih-Hui Chao, Mark R Holl, Sarah C McQuaide

|June 24, 2009

Summary

This summary is machine-generated.

A novel digital light modulation microscope (DLMM) enables phosphorescence lifetime detection for oxygen (O2) sensing without pulsed light or time-gated cameras. This technology allows for precise O2 mapping using microsensor arrays and lab-on-a-chip devices.

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Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Published on: March 3, 2010

Area of Science:

Microscopy and Imaging
Biomedical Engineering
Sensor Technology

Background:

Phosphorescence lifetime imaging is crucial for oxygen sensing.
Conventional methods require specialized pulsed light sources and time-gated cameras.
Developing simpler, more accessible O2 detection methods is needed.

Purpose of the Study:

To develop a digital light modulation microscope (DLMM) for phosphorescence lifetime detection.
To enable spatial and temporal modulation of excitation light for enhanced O2 sensing.
To create a platform for O2 consumption measurement in individual cells.

Main Methods:

Utilized a digital micromirror device (DMD) integrated into an epifluorescence microscope.
Employed phosphorescent porphyrin microsensors for O2 detection based on lifetime changes.
Developed image analysis for automatic identification of sensor elements in micro-well arrays.

Main Results:

Successfully demonstrated O2 sensing using DLMM with phosphorescent porphyrin-coated micro-wells.
Achieved discrete lifetime imaging and O2 distribution mapping.
Eliminated the need for pulsed light sources and time-gated cameras.

Conclusions:

The DLMM offers a simplified and effective approach to phosphorescence lifetime-based O2 sensing.
This technology is suitable for lab-on-a-chip devices and measuring cellular O2 consumption.
The DLMM platform advances high-resolution O2 mapping capabilities.