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Related Concept Videos

Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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Related Experiment Video

Updated: Jun 22, 2026

A Protocol for Using Gene Set Enrichment Analysis to Identify the Appropriate Animal Model for Translational Research
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Published on: August 16, 2017

[CENTB5 gene expression in human and mouse].

D M Shubina, I O Suchkova, N A Slominskaia

    Molekuliarnaia Biologiia
    |June 25, 2009
    PubMed
    Summary
    This summary is machine-generated.

    The minisatellite UPS29 in the CENTB5 gene may regulate expression of CENTB5, SCNN1D, and ACOT7. This finding could link genetic variations to neurological disorders like Parkinson disease and epilepsy.

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    Cell Type-specific Gene Expression Profiling in the Mouse Liver
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    Cell Type-specific Gene Expression Profiling in the Mouse Liver

    Published on: September 17, 2019

    Area of Science:

    • Genetics
    • Neuroscience
    • Molecular Biology

    Context:

    • Centaurin beta5 (CENTB5) is a protein of unknown function within the centaurin family.
    • Human CENTB5 gene's intron contains the minisatellite UPS29; mouse homolog has a microsatellite repeat.
    • CENTB5, SCNN1D, and ACOT7 genes are located in the same syntenic group and are expressed in the nervous system, with mutations linked to neurodegenerative diseases and epilepsy.

    Purpose:

    • To investigate the potential regulatory role of intra-intronic minisatellite UPS29 and microsatellite (CATG)19 on the expression of CENTB5, SCNN1D, and ACOT7.
    • To analyze the expression patterns of CENTB5, SCNN1D, and ACOT7 across various tissues using RT-PCR.

    Summary:

    • RT-PCR analysis confirmed expression of CENTB5, SCNN1D, and ACOT7 in all studied organs and tissues.
    • In silico evaluation suggested that the intra-intronic tandem repeats UPS29 (human) and (CATG)19 (mouse) have the potential to regulate gene activity.
    • It is hypothesized that the minisatellite locus UPS29 can modulate the activity of CENTB5, SCNN1D, and ACOT7 within nervous system cells.

    Impact:

    • Provides a potential molecular mechanism linking minisatellite variations to neurological conditions.
    • Highlights the regulatory role of non-coding DNA elements, specifically intra-intronic repeats, in gene expression.
    • Suggests novel targets for understanding and potentially treating neurodegenerative diseases and epilepsy associated with these gene loci.