Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 22, 2026

Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability
09:49

Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability

Published on: April 2, 2015

Salt effects on surface-tethered peptides in solution.

Jun Feng1, Ka-Yiu Wong, Gillian C Lynch

  • 1Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204-5001, USA.

The Journal of Physical Chemistry. B
|June 25, 2009
PubMed
Summary

This study used molecular dynamics simulations to investigate peptide behavior on surfaces. Surface tethering and salt concentration affect peptide thermodynamics and kinetics, slowing conformational changes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of Probe-Related Correlations on Local Electrostatic Potentials Around DNA.

Journal of computational chemistry·2025
Same author

Murine norovirus allosteric escape mutants mimic gut activation.

Journal of virology·2025
Same author

Strong field gradients enable NMR-based diffusion measurements for K<sup>+</sup>, Mg<sup>2+</sup>, Cl<sup>-</sup>, and SO<sub>4</sub><sup>2-</sup> ions in biomolecular solutions.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2025
Same author

The need to implement FAIR principles in biomolecular simulations.

Nature methods·2025
Same author

Murine norovirus allosteric escape mutants mimic gut activation.

bioRxiv : the preprint server for biology·2025
Same author

Peptide diffusion in biomolecular condensates.

Biophysical journal·2024

Area of Science:

  • Biophysics
  • Surface Chemistry
  • Computational Biology

Background:

  • Protein and peptide manipulation at liquid-solid interfaces is crucial for biotechnology and detector applications.
  • Understanding the molecular behavior of surface-tethered peptides is essential due to surface inhomogeneity and ion effects.
  • Peptides and proteins exhibit unique thermodynamic and kinetic properties when interacting with hard material surfaces.

Purpose of the Study:

  • To investigate the molecular dynamics of a pentapeptide (RHSVV) tethered to a microarray surface.
  • To analyze the influence of varying salt concentrations (0–1 M NaCl) on tethered and free peptides.
  • To compare the local ion composition around tethered versus free peptides.

Main Methods:

  • Molecular dynamics simulations were employed.

More Related Videos

Study of Short Peptide Adsorption on Solution Dispersed Inorganic Nanoparticles Using Depletion Method
09:43

Study of Short Peptide Adsorption on Solution Dispersed Inorganic Nanoparticles Using Depletion Method

Published on: April 11, 2020

Glycopeptide Capture for Cell Surface Proteomics
10:11

Glycopeptide Capture for Cell Surface Proteomics

Published on: May 9, 2014

Related Experiment Videos

Last Updated: Jun 22, 2026

Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability
09:49

Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability

Published on: April 2, 2015

Study of Short Peptide Adsorption on Solution Dispersed Inorganic Nanoparticles Using Depletion Method
09:43

Study of Short Peptide Adsorption on Solution Dispersed Inorganic Nanoparticles Using Depletion Method

Published on: April 11, 2020

Glycopeptide Capture for Cell Surface Proteomics
10:11

Glycopeptide Capture for Cell Surface Proteomics

Published on: May 9, 2014

  • Simulations included a pentapeptide tethered to a surface and free in solution.
  • Experiments were conducted across a range of NaCl concentrations (0, 0.14, 0.5, and 1 M).

    • Main Results:

    • Tethered peptide conformational space largely overlaps with free peptides in solution.
    • Surface tethering and salt concentration significantly alter peptide thermodynamics and kinetics.
    • Increased salt concentration and surface tethering reduce the frequency of peptide conformational changes.

    Conclusions:

    • Surface tethering and ionic strength modulate peptide dynamics.
    • The study provides insights into peptide-surface interactions relevant for biosensor and biotechnology design.
    • Conformational dynamics of tethered peptides are sensitive to their ionic environment.