Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Clinical Applications of Epidermal Stem Cells

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own EpiSCs...

Renewal of Skin Epidermal Stem Cells

Renewal of Skin Epidermal Stem Cells

The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...

Stem Cell Culture

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...

Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.

Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Comment on 'De novo generalized pustular psoriasis following Oxford-AstraZeneca COVID-19 vaccine': possible role for Type I interferons.

Clinical and experimental dermatology·2021

Same author

Comment on 'Vitiligo in a COVID-19-vaccinated patient with ulcerative colitis: coincidence?': Type I interferons as possible link between COVID-19 vaccine and vitiligo.

Clinical and experimental dermatology·2021

Same author

Genetic profile of naïve untreated primary cutaneous melanomas with a negative sentinel lymph node.

Clinical and experimental dermatology·2020

Same author

Tuberous sclerosis with two unusual findings: cutaneous rhabdomyoma and chronic myelogenous leukaemia.

Clinical and experimental dermatology·2020

Same author

Chanarin-Dorfman syndrome: a novel homozygous mutation in the ABHD5 gene.

Clinical and experimental dermatology·2019

Same author

Temperature dependent Raman and X-ray diffraction studies of anhydrous milk fat.

Food chemistry·2018

Search research articles

Related Experiment Video

Updated: Jun 22, 2026

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin

Published on: March 31, 2011

Epidermal stem cells: practical perspectives and potential uses.

O Abbas¹, M Mahalingam

¹Department of Dermatology, American University of Beirut Medical Center, Beirut, Lebanon.

The British Journal of Dermatology

|June 25, 2009

Summary

This review explores skin stem cell markers for understanding epidermal homeostasis, hair regeneration, and skin injury repair. Identifying these stem cell niches aids in diagnosing and treating hair loss and skin cancers.

More Related Videos

Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity

Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity

Published on: January 30, 2021

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

Published on: April 29, 2016

Related Experiment Videos

Last Updated: Jun 22, 2026

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin

Published on: March 31, 2011

Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity

Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity

Published on: January 30, 2021

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

Published on: April 29, 2016

Area of Science:

Dermatology and Stem Cell Biology
Investigates the role of stem cells in skin and hair follicle biology.

Background:

The epidermis and hair follicle continuously cycle through growth, regression, and rest.
Epidermal stem cells are crucial for maintaining skin homeostasis, hair regeneration, and wound repair.
Stem cells reside in specific niches within the hair follicle and epidermis.

Purpose of the Study:

To review potential markers for identifying and studying skin stem cells.
To enhance understanding of the pathogenesis of skin diseases, including hair loss and malignancies.
To explore the utility of these markers in prognosis and therapeutic strategies.

Main Methods:

Utilizes monoclonal antibodies for identifying stem cell niches in formalin-fixed paraffin-embedded tissues.
Reviews existing literature on stem cell markers in dermatological research.
Focuses on methodologies facilitating stem cell niche discovery.

Main Results:

Highlights the importance of specific markers in delineating stem cell populations.
Discusses the role of stem cell niches in maintaining skin structure and function.
Emphasizes the potential for marker-based diagnostics and therapeutics.

Conclusions:

Potential markers are key to understanding skin stem cell biology and related diseases.
Improved identification of stem cells can lead to better diagnostic and therapeutic approaches for skin disorders.
Further research into stem cell markers promises advancements in treating conditions like hair loss and skin cancer.