Related Experiment Video
Updated: Jun 22, 2026

Multiplex Detection of Gene Expression in the Intact Drosophila Brain Using Expansion-Assisted Iterative Fluorescence In Situ Hybridization
Published on: May 2, 2025
GCPReg package for registration of the segmentation gene expression data in Drosophila
Konstantin N Kozlov1, Ekaterina Myasnikova, Anastasia A Samsonova
1Department of Computational Biology, Center for Advanced Studies, St. Petersburg State Polytechnical University, St. Petersburg, Russia.
Abstract:
In modern functional genomics registration techniques areused to construct reference gene expression patterns and createa spatiotemporal atlas of the expression of all the genes in anetwork. In this paper we present a software package calledGCPReg, which can be used to register the expression patterns ofsegmentation genes in the early Drosophila embryo. The key task,which this package performs, is the extraction of spatially localizedcharacteristic features of expression patterns. To facilitatethis task, we have developed an easy-to-use interactive graphicalinterface. We describe GCPReg usage and demonstrate how thispackage can be applied to register gene expression patterns inwild type and mutants. GCPReg has been designed to operate ona UNIX platform and is freely available via the Internet at http://urchin.spbcas.ru/downloads/GCPReg/GCPReg.htm.

