Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.
Atomic Absorption Spectroscopy: Instrumentation01:22

Atomic Absorption Spectroscopy: Instrumentation

An atomic absorption spectrophotometer (AAS) comprises several components: a radiation source, an atomizer, a monochromator, and a detector. The radiation source can be a hollow-cathode lamp (HCL) or an electrodeless-discharge lamp (EDL), both of which provide a narrow emission line of the required wavelength. However, some instruments use continuum sources and high-resolution monochromators to achieve a narrow range of radiation.
The atomizer used in AAS can be either a flame atomizer or an...
UV–Vis Spectroscopy of Conjugated Systems01:32

UV–Vis Spectroscopy of Conjugated Systems

Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π → π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
One of the factors influencing λmax is the extent of conjugation in the...
Atomic Emission Spectroscopy: Instrumentation01:22

Atomic Emission Spectroscopy: Instrumentation

The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acute and Chronic Myocarditis in Men and Women.

Current heart failure reports·2026
Same author

Vacuolar and ER-Ca2+-ATPases regulate calcium dynamics during pollen tube growth in Arabidopsis thaliana.

Plant physiology·2026
Same author

New Perspectives Provided by Merging Computed Tomographic Scanning and Electroanatomical Mapping of Koch's Pyramid.

Journal of cardiovascular development and disease·2026
Same author

Computational 3D multispectral fluorescence lifetime microscopy.

Optics express·2026
Same author

Phantom for standardization in functional near-infrared spectroscopy, part 2: optical properties and Monte Carlo simulations.

Neurophotonics·2026
Same author

Amplitude- and Phase-Programmable Dual-Color Photonic Chip for High-Contrast Structured Illumination Microscopy.

ACS photonics·2026
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Jun 22, 2026

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
10:03

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Published on: June 27, 2014

Portable, large-bandwidth time-resolved system for diffuse optical spectroscopy.

Andrea Bassi, Andrea Farina, Cosimo D'Andrea

    Optics Express
    |June 25, 2009
    PubMed
    Summary
    This summary is machine-generated.

    We developed a compact system to measure optical properties of scattering media using supercontinuum light and time-correlated single-photon counting. This tool accurately analyzes tissue optical properties, validated with phantoms and in-vivo breast measurements.

    More Related Videos

    High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis
    13:31

    High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis

    Published on: December 22, 2015

    Real-Time Monitoring of Neurocritical Patients with Diffuse Optical Spectroscopies
    07:12

    Real-Time Monitoring of Neurocritical Patients with Diffuse Optical Spectroscopies

    Published on: November 19, 2020

    Related Experiment Videos

    Last Updated: Jun 22, 2026

    Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
    10:03

    Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

    Published on: June 27, 2014

    High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis
    13:31

    High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis

    Published on: December 22, 2015

    Real-Time Monitoring of Neurocritical Patients with Diffuse Optical Spectroscopies
    07:12

    Real-Time Monitoring of Neurocritical Patients with Diffuse Optical Spectroscopies

    Published on: November 19, 2020

    Area of Science:

    • Biomedical Optics
    • Photonics
    • Medical Imaging

    Background:

    • Accurate measurement of optical properties is crucial for understanding light transport in scattering media.
    • Existing methods may lack compactness or a broad spectral range for comprehensive analysis.

    Purpose of the Study:

    • To develop and validate a compact, time-resolved system for measuring optical properties of highly scattering media.
    • To cover a broad spectral bandwidth (600-1000 nm) for versatile applications.

    Main Methods:

    • Utilized a fiber laser generating supercontinuum radiation, spectrally dispersed for sample illumination.
    • Employed a single photon avalanche photodiode with time-correlated single-photon counting.
    • Applied the diffusion equation to fit time-dispersion curves at each wavelength.

    Main Results:

    • Successfully developed a compact time-resolved optical properties measurement system.
    • Demonstrated accurate measurements on calibrated epoxy phantoms.
    • Validated in-vivo performance through transmittance measurements on female breast tissue.

    Conclusions:

    • The developed system offers a robust and compact solution for characterizing optical properties of scattering media.
    • The system's accuracy and broad spectral range make it suitable for various biomedical and optical applications.
    • Results show good agreement with established literature, confirming the system's reliability.