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Guided selection methods through chain shuffling

Bao Guo-Qiang1, He Xian-Li

  • 1Department of General Surgery, Tangdu Hospital, Xi'an 710038, Shaanxi Province, China. guoqiang_bao@hotmail.com

Methods in Molecular Biology (Clifton, N.J.)
|June 26, 2009
PubMed

We provide procedures for the panning of fully humanized Fab antibodies using guided selection. Human heavy and light chain genes are amplified. A parental light chain is cloned into a phage display vector and combined with the heavy chain library. After several rounds of panning, positive clones are identified and the heavy chain sequences that are recovered are combined with light chains for further selection by phage display. Human Fab antibodies are obtained that bind the same epitope as the parental antibody.

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