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Updated: Jun 22, 2026
Protein Engineering by Yeast Surface Display
Published on: November 29, 2024
Identification of the specificity of isolated phage display single-chain antibodies using yeast two-hybrid screens
Nicolaj Rasmussen1, Henrik J Ditzel
1Institute of Medical Biology, University of Southern Denmark, Winsloewparken 25, 3, DK-5000, Odense, Denmark.
A method is described for the identification of the antigen recognised by an scFv isolated from an antibody phage display library using selection against a complex mixture of proteins (e.g. intact cells, purified cell surface membranes, and tissue sections). The method takes advantage of a yeast two-hybrid system that additionally allows for reorganization of post-translational modifications to the bait and target proteins. This technique is therefore especially useful for identifying surface-expressed antigens.
A method is described for the identification of the antigen recognised by an scFv isolated from an antibody phage display library using selection against a complex mixture of proteins (e.g. intact cells, purified cell surface membranes, and tissue sections). The method takes advantage of a yeast two-hybrid system that additionally allows for reorganization of post-translational modifications to the bait and target proteins. This technique is therefore especially useful for identifying surface-expressed antigens.
