Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
Chromosome Replication02:31

Chromosome Replication

Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin of...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Single Trophoblast Layer Acts as the Gatekeeper at the Endothelial-Hematopoietic Crossroad in the Placenta.

bioRxiv : the preprint server for biology·2024
Same author

METTL3 shapes m6A epitranscriptomic landscape for successful human placentation.

bioRxiv : the preprint server for biology·2024
Same author

Global chromatin organizer SATB1 acts as a context-dependent regulator of the Wnt/Wg target genes.

Scientific reports·2021
Same author

The Hippo Pathway Prevents YAP/TAZ-Driven Hypertranscription and Controls Neural Progenitor Number.

Developmental cell·2018
Same author

Genetic redundancy of GATA factors in the extraembryonic trophoblast lineage ensures the progression of preimplantation and postimplantation mammalian development.

Development (Cambridge, England)·2017
Same author

Repeat performance: how do genome packaging and regulation depend on simple sequence repeats?

BioEssays : news and reviews in molecular, cellular and developmental biology·2010

Related Experiment Video

Updated: Jun 22, 2026

A Method to Study de novo Formation of Chromatin Domains
07:34

A Method to Study de novo Formation of Chromatin Domains

Published on: August 23, 2019

Polycomb group proteins: remembering how to catch chromatin during replication.

Ram Parikshan Kumar1

  • 1Centre for Cellular and Molecular Biology, Hyderabad, India. ramparik@ccmb.res.in

Bioessays : News and Reviews in Molecular, Cellular and Developmental Biology
|June 26, 2009
PubMed
Summary

Polycomb group (PcG) proteins and H3K27me3 modifications help maintain gene expression states during development. Both PcG proteins and histone modifications appear to be propagated through DNA replication.

More Related Videos

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
09:14

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique

Published on: January 14, 2016

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Related Experiment Videos

Last Updated: Jun 22, 2026

A Method to Study de novo Formation of Chromatin Domains
07:34

A Method to Study de novo Formation of Chromatin Domains

Published on: August 23, 2019

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
09:14

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique

Published on: January 14, 2016

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Area of Science:

  • Epigenetics and Gene Regulation
  • Chromatin Biology
  • Developmental Biology

Background:

  • Polycomb group (PcG) proteins are crucial for maintaining the expression status of PcG-responsive genes throughout multicellular organism development.
  • The H3K27me3 modification is a key epigenetic mark associated with gene silencing by Polycomb repressive complexes (PRCs).

Purpose of the Study:

  • To investigate the mechanisms by which Polycomb group proteins and their associated histone modifications are maintained across DNA replication.
  • To explore the role of H3K27me3 in the propagation of Polycomb repressive complex 2 (PRC2) on replicating chromatin.
  • To examine the potential for Polycomb repressive complex 1 (PRC1) to be maintained on replicating chromatin independently of H3K27me3.

Main Methods:

  • Review of recent observations and in vitro studies concerning Polycomb group protein and H3K27me3 maintenance during DNA replication.
  • Analysis of the interplay between histone modifications and PcG protein binding on newly replicated chromatin.

Main Results:

  • Evidence suggests H3K27me3 modification helps anchor PRC2 to replicating chromatin, facilitating its propagation to daughter strands.
  • PRC1-class complexes may also be maintained on replicating chromatin, potentially independent of the H3K27me3 mark.
  • Both histone modifications and PcG proteins themselves appear to be stably inherited through the replication process.

Conclusions:

  • Polycomb group proteins and H3K27me3 modifications play a coordinated role in ensuring epigenetic memory through DNA replication.
  • This maintenance mechanism is vital for preserving developmental gene expression patterns in proliferating cells.