Related Experiment Video
Updated: Jun 22, 2026

Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization
Published on: April 5, 2019
Electrochromatographic evaluation of diol-bonded silica monolith capillary column for separation of basic compounds
Fanggui Ye1, Shuting Li, Shulin Zhao
1College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541004, China.
Abstract:
A diol-bonded silica monolith capillary column as polar stationary phase was successfully prepared for capillary electrochromatography. The preparation of monolithic stationary phase was based on the individual silica matrix forming and subsequent chemical bonding by (3-glycidoxypropyl) trimethoxysilane to produce the desired function. The diol-bonded silica monolith has been successfully employed in the electrochromatographic separation of alkaloids. The effects of experimental parameters, such as the volume fraction of the organic modifier, pH value, and ionic strength of the buffer on the retention behavior of the solutes were investigated. Column efficiencies greater than 110,000 plates/m for separation of basic compounds were obtained. It was observed that retention of alkaloids on the diol-bonded silica monolith was mainly contributed to a reversed-phase and cation-exchange mechanism, and electrophoresis of basic compounds also played a role in separation.
More Related Videos
09:34Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry
Published on: September 20, 2016
07:54Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
Published on: October 27, 2020
Related Concept Videos
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Silica Gel Column Chromatography: Overview
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
Ion-Exchange Chromatography
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Electrophoresis: Overview
There...
Gas Chromatography: Types of Columns and Stationary Phases
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...