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Related Concept Videos

Lysosomal Hydrolases01:22

Lysosomal Hydrolases

Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Lysosomes01:31

Lysosomes

Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules and biological polymers that are released during membrane trafficking events such as the secretory, endocytic, autophagic, and phagocytic pathways. The degradation is carried out by several hydrolytic enzymes active in an acidic environment of the lysosomal lumen. These acid hydrolases are involved in cellular processes such as cell signaling,...
Lysosomes01:31

Lysosomes

Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules and biological polymers that are released during membrane trafficking events such as the secretory, endocytic, autophagic, and phagocytic pathways. The degradation is carried out by several hydrolytic enzymes active in an acidic environment of the lysosomal lumen. These acid hydrolases are involved in cellular processes such as cell signaling,...
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...

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Related Experiment Video

Updated: Jun 22, 2026

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
11:40

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

A gene network regulating lysosomal biogenesis and function.

Marco Sardiello1, Michela Palmieri, Alberto di Ronza

  • 1Telethon Institute of Genetics and Medicine, Via P. Castellino 111, 80131 Naples, Italy.

Science (New York, N.Y.)
|June 27, 2009
PubMed
Summary
This summary is machine-generated.

Cellular lysosomes coordinate their activity through the transcription factor EB (TFEB). TFEB activation enhances lysosome function, offering a therapeutic target for storage and neurodegenerative diseases.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Lysosomes are crucial for cellular degradation and recycling.
  • The coordination of lysosomal activity in response to cellular needs is not well understood.

Purpose of the Study:

  • To investigate the coordination of lysosomal gene expression.
  • To identify regulatory mechanisms governing lysosomal activity.

Main Methods:

  • Analysis of lysosomal gene expression patterns.
  • Investigating the role of transcription factor EB (TFEB) in lysosomal regulation.
  • Cellular assays to assess lysosomal biogenesis and degradation capacity.

Main Results:

  • Most lysosomal genes show coordinated transcriptional regulation.
  • TFEB acts as a key regulator, translocating to the nucleus under stress.
  • TFEB activation boosts lysosomal biogenesis and enhances the degradation of molecules like glycosaminoglycans and pathogenic proteins.

Conclusions:

  • A genetic program, orchestrated by TFEB, controls lysosomal biogenesis and function.
  • This TFEB-mediated pathway presents a potential therapeutic strategy for lysosomal storage and neurodegenerative diseases.