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Published on: March 8, 2012
Development and validation of a HPV-32 specific PCR assay
Nicholas R Herrel1, Nadia L Johnson, Jennifer E Cameron
1Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, USA. nherre@lsuhsc.edu
Virology Journal
|June 30, 2009
Summary
A new Human Papillomavirus-32 (HPV-32) polymerase chain reaction (PCR) assay offers superior sensitivity and ease of use for detecting HPV-32. This advanced PCR method is ideal for large-scale studies on oral warts and HPV-32 infection natural history.
Area of Science:
- Virology
- Molecular Biology
- Infectious Diseases
Background:
- Human Papillomavirus-32 (HPV-32) is linked to focal epithelial hyperplasia and oral warts in HIV-positive individuals.
- Increasing prevalence of HPV-32 necessitates improved detection methods.
- Current HPV-32 detection techniques are labor-intensive and lack sensitivity.
Purpose of the Study:
- To develop a highly sensitive and user-friendly specific polymerase chain reaction (PCR) assay for HPV-32 detection.
- To overcome the limitations of existing HPV-32 detection methods.
Main Methods:
- Developed and optimized an HPV-32 L1 specific PCR assay.
- Compared its sensitivity and specificity against PGMY and MY09/11 PCR with dot blot hybridization.
- Utilized cloned HPV-32 L1, HPV-42 L1, and clinical samples from 89 HIV-positive subjects.
Main Results:
- The new HPV-32 specific PCR assay detected as few as 5 copies of HPV-32, a significant improvement over the ~3,000 copy detection limit of previous methods.
- Achieved 95.8% sensitivity and 87.8% specificity by sample (88.9% sensitivity, 58.8% specificity by subject) when compared to the HPV-32 dot blot hybridization assay.
- The assay's high sensitivity may establish it as a new gold standard for HPV-32 detection.
Conclusions:
- The developed HPV-32 specific PCR assay is superior to existing methods due to its ease of use, high sensitivity, and specificity.
- This assay is well-suited for detecting HPV-32 in large populations.
- It serves as a valuable tool for investigating HPV-32 infection and oral wart development.
