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Related Experiment Video

Updated: Jun 22, 2026

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

Comparative transcriptomics in the Triticeae.

Andreas W Schreiber¹, Tim Sutton, Rico A Caldo

¹Australian Centre for Plant Functional Genomics, Univ of Adelaide, PMB 1 Glen Osmond, SA 5064, Australia. andreas.schreiber@adelaide.edu.au

|June 30, 2009

Summary

This summary is machine-generated.

Wheat and barley transcriptomes show high correlation, demonstrating the utility of comparative genomics. Gene sequence and expression conservation are linked to expression levels in Triticeae species.

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Area of Science:

Plant genomics
Comparative transcriptomics
Agricultural biotechnology

Background:

Barley and wheat are agriculturally vital, closely related grass species.
Polyploidy in wheat does not prevent fertile hybridization with barley.
Genome-scale Affymetrix GeneChips enable transcriptome comparison.

Purpose of the Study:

To create a gene expression atlas for wheat (cv. Chinese Spring) using the Wheat GeneChip.
To perform a genomic-scale comparative study of wheat and barley transcriptomes.
To explore wheat polyploidy's influence on gene expression and sequence conservation.

Main Methods:

Utilized Wheat GeneChip and Barley1 GeneChip for transcriptome analysis.
Created mRNA expression atlas from diverse wheat tissues and developmental stages.
Clustered probesets into homologous groups for cross-species comparison.

Main Results:

Wheat and barley transcriptomes exhibit high correlation despite individual gene/tissue expression differences.
Demonstrated feasibility of comparative transcriptomics using distinct GeneChip designs.
Wheat GeneChip generally cannot distinguish homoeolog contributions.

Conclusions:

Comparative transcriptomics between wheat and barley is feasible and reveals high correlation.
Sequence and expression conservation correlate positively with absolute expression levels.
Evidence suggests a correlation between sequence and expression conservation within Triticeae.