Related Experiment Video
Updated: Jun 22, 2026
Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment
Published on: April 22, 2019
Molecular markers of micrometastasis in oral cavity carcinomas
Ross M Germani1, Francisco J Civantos, George Elgart
1Department of Otolaryngology, University of Miami, Miami, FL 33136, USA. rgermani@med.miami.edu
Objective:
To examine the expression of candidate markers for micrometastasis.
Study Design:
Cross-sectional analysis of subjects with oral cavity carcinomas who underwent sentinel lymph node biopsy (SLNB) and subsequent immunohistochemical (IHC) analysis.
Subjects And Methods:
Two groups were identified based on SLNB status: negative SLNB (19/30) and positive SLNB (11/30). Specimens underwent IHC using conjugated monoclonal antibodies for membrane type-1 matrix metalloproteinase (MT1-MMP), CD44, focal adhesion kinase-1, and E-cadherin. Staining results were evaluated to determine if a particular marker was associated with SLNB status or other histopathologic prognosticators.
Results:
For MT1-MMP, 21 percent (3/14) of evaluable specimens stained positively in the SLNB(-) group and 67 percent (4/6) stained positively in the SLNB(+) group (P=0.12). No statistically significant association was seen between any marker's staining pattern and SLNB status alone. When MT1-MMP staining was evaluated in tumors with SLNB(+) or perineural invasion (PNI) present on histopathology, six of nine specimens (67%) stained positively for MT1-MMP, vs one of 11 (9%) in specimens lacking either negative prognosticator (P=0.016, RR=7.33).
Conclusion:
Preliminary results suggest that MT1-MMP positivity in primary tumor specimens may identify aggressive tumor types, evidenced by the presence of micrometastasis or PNI.
