Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 22, 2026

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

Published on: April 7, 2016

Gene delivery to cornea.

Jinsong Hao¹, S Kevin Li, Winston W Y Kao

¹Division of Pharmaceutical Sciences, College of Pharmacy, University of Cincinnati, Cincinnati, OH 45267, USA.

Brain Research Bulletin

|June 30, 2009

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Lateral Transport in Anisotropic Membrane during Permeation Study in Diffusion Cell.

Pharmaceutical research·2026

Same author

Generation of a Novel Col8a2<sup>P2A-CreERT2</sup> Mouse Line Enables Targeted Genetic Manipulation of Corneal Endothelial Cells and Modeling of Endothelial Decompensation.

Genesis (New York, N.Y. : 2000)·2026

Same author

Rheological Characterization of Lemon Oil-Loaded Eucerin Cream and Aquaphor Ointment.

Pharmaceuticals (Basel, Switzerland)·2025

Same author

2025 Top JPharmSci Reviewers.

Journal of pharmaceutical sciences·2025

Same author

Targeting the Eye: RNA-Based Therapies, Interferences, and Delivery Strategies.

Pharmaceutics·2025

Same author

Co-delivery of ripasudil and dexamethasone in trabecular meshwork cells for potential prevention of GC-induced ocular hypertension.

Experimental cell research·2025

This review explores in vivo gene delivery methods for corneal cells. Strategies like intrastromal injection and gene gun show promise for delivering genetic material to stromal keratocytes and epithelial cells.

Area of Science:

Ophthalmology
Gene Therapy
Biotechnology

Background:

Corneal gene therapy holds potential for treating various eye diseases.
Efficient and targeted delivery of genetic material to corneal cells remains a challenge.

Purpose of the Study:

To review and summarize current strategies for in vivo gene delivery to the cornea.
To evaluate the efficacy of different delivery methods for various genetic materials to specific corneal cell types.

Main Methods:

Review of existing literature on in vivo corneal gene delivery techniques.
Analysis of delivery methods including intrastromal injection, iontophoresis, electroporation, and gene gun.
Assessment of delivery of oligonucleotides, small interfering RNA (siRNA), plasmid DNA, and viral vectors.

More Related Videos

An “All-laser” Endothelial Transplant

An “All-laser” Endothelial Transplant

Published on: July 6, 2015

Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium

Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium

Published on: August 7, 2015

Related Experiment Videos

Last Updated: Jun 22, 2026

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

Published on: April 7, 2016

An “All-laser” Endothelial Transplant

An “All-laser” Endothelial Transplant

Published on: July 6, 2015

Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium

Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium

Published on: August 7, 2015

Main Results:

Intrastromal injection effectively delivers plasmid DNA and adenovirus to stromal keratocytes.
Gene gun is effective for delivering genes to corneal epithelium by disrupting barriers.
Iontophoresis and electroporation successfully deliver siRNA, but not plasmid DNA, to corneal epithelium.
Nanocarriers, such as polymeric micelles, show promise for corneal gene delivery.

Conclusions:

Various methods exist for in vivo corneal gene delivery, with varying efficacy for different cell types and genetic materials.
Targeting corneal epithelium requires more invasive methods compared to stromal keratocytes.
Nanocarrier-based approaches represent a promising future direction for corneal gene therapy.