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Related Experiment Video

Updated: Jun 22, 2026

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

Published on: June 23, 2016

Adenosine detection by using gold nanoparticles and designed aptamer sequences.

Fan Li¹, Juan Zhang, Xuni Cao

¹State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai, 200237, China.

|June 30, 2009

Summary

This summary is machine-generated.

Researchers developed a new gold nanoparticle (AuNP) and DNA aptamer bioassay for detecting adenosine. This method uses aptamer reassembly on AuNPs to signal the presence of adenosine, enabling selective detection.

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Area of Science:

Nanotechnology
Biotechnology
Analytical Chemistry

Background:

Gold nanoparticles (AuNPs) offer unique optical properties for biosensing.
DNA aptamers are versatile recognition elements for small molecules.
Developing sensitive and selective assays for small molecules like adenosine is crucial.

Purpose of the Study:

To design a novel bioassay strategy for adenosine detection using AuNPs and engineered DNA aptamers.
To leverage aptamer reassembly and AuNP aggregation for signal generation.
To demonstrate the selectivity and sensitivity of the developed assay.

Main Methods:

Engineered DNA aptamers composed of two ssDNA pieces were synthesized.
Aptamers were attached to the 5'-thiol-modified end of AuNPs.
Adenosine binding induced aptamer reassembly and AuNP aggregation.
Surface plasmon resonance (SPR)-based color change of AuNPs was monitored.

Main Results:

The bioassay successfully detected adenosine in the low micromolar range.
The method demonstrated high selectivity for adenosine.
The characteristic SPR-based color change of AuNPs effectively indicated adenosine presence.
The assay design proved effective in differentiating DNA structures.

Conclusions:

A novel and effective bioassay strategy for adenosine detection was established using AuNPs and DNA aptamers.
The aptamer reassembly-induced AuNP aggregation mechanism provides a sensitive detection platform.
This strategy holds potential for the detection of various other small target molecules.