Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Bioreactor Controls-III01:22

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamic nomogram for predicting axillary lymph node metastasis in breast cancer based on MRI analysis and the platelet-to-lymphocyte ratio (PLR).

Cancer imaging : the official publication of the International Cancer Imaging Society·2026
Same author

A triple-emission ratiometric fluorescence and multi-color sensing platform for the detection of 6PPD-Q based on aggregation-induced emission.

Mikrochimica acta·2026
Same author

Mindfulness Meditation Combined with eCASH-Based Nursing for Cesarean Section in Preeclampsia: A Retrospective Study on Perioperative Pain and Recovery.

International journal of women's health·2026
Same author

Quantum dots and cationic carrier-reinforced electromembrane extraction of typical illegal dyes in fish samples followed by capillary electrophoresis with contactless conductivity detection.

Journal of the science of food and agriculture·2026
Same author

Thermostable Chaperone Tag Fusion <i>In Vitro</i>: Development of TST-TE Complexes for Enhanced Enzyme Thermal Stability.

Journal of agricultural and food chemistry·2026
Same author

Rational fabrication of molecularly imprinted polymers based ratiometric fluorescence sensing platform with aggregation-induced emission effect for visual detection of 6PPD-Q in aquatic products.

Food chemistry·2026
Same journal

Developing Anti-EGFR/Anti-HER2 Bifunctional Antibody for Solid Tumors by Protein Engineering.

Biotechnology and bioengineering·2026
Same journal

Bridging Organ-on-a-Chip and Omics: A Multi-Dimensional Frontier in Biomedical Research.

Biotechnology and bioengineering·2026
Same journal

Hemopexin Purification From Human Cohn Fraction IV Paste and Its Biophysical Characterization and Functional Evaluation in Sickle Cell Disease Mice.

Biotechnology and bioengineering·2026
Same journal

Characterization and Therapeutic Potential of a Novel Lytic Phage-Derived Endolysin PA16cLys Against Uropathogenic Pseudomonas aeruginosa Biofilms.

Biotechnology and bioengineering·2026
Same journal

Modeling Multiscale Architecture of Biofilm Extracellular Matrix and Its Role in Oxygen Transport.

Biotechnology and bioengineering·2026
Same journal

A Behavior-Integrated Potency Assay for Quantitative Evaluation of Extracellular Matrix Remodeling by Mesenchymal Stem/Stromal Cells.

Biotechnology and bioengineering·2026
See all related articles

Related Experiment Video

Updated: May 13, 2026

High-throughput Yeast Plasmid Overexpression Screen
08:57

High-throughput Yeast Plasmid Overexpression Screen

Published on: July 27, 2011

16.5K

Modular RNAi Pathway Engineering Enhances Plasmid Copy Number Control in Yeast Bioproduction System.

Qianru Cai1, Manman Wang1, Jinmei Zhu1

  • 1Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou, Zhejiang, China.

Biotechnology and Bioengineering
|June 30, 2025
PubMed
Summary
This summary is machine-generated.

Scientists engineered a dynamic plasmid copy number system in yeast using RNA interference (RNAi). This synthetic biology tool boosts gene dosage control and enhances microbial cell factory production.

Keywords:
RNAidynamic controlplasmid copy numbersiRNA

More Related Videos

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing
07:55

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

Published on: May 21, 2020

7.1K
Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

13.9K

Related Experiment Videos

Last Updated: May 13, 2026

High-throughput Yeast Plasmid Overexpression Screen
08:57

High-throughput Yeast Plasmid Overexpression Screen

Published on: July 27, 2011

16.5K
High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing
07:55

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

Published on: May 21, 2020

7.1K
Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

13.9K

Area of Science:

  • Synthetic Biology
  • Metabolic Engineering
  • Molecular Biology

Background:

  • Optimizing metabolic flux in microbial cell factories requires precise control over gene dosage and expression dynamics.
  • Cellular burden must be minimized to ensure efficient production.
  • Dynamic regulation of gene expression is crucial for advanced metabolic engineering.

Purpose of the Study:

  • To develop a synthetic biology chassis in Saccharomyces cerevisiae with dynamically programmable plasmid copy numbers.
  • To establish a chemically inducible platform for precise gene dosage control.
  • To enhance the production of valuable compounds in microbial cell factories.

Main Methods:

  • Integrated heterologous RNA interference (RNAi) pathway genes from Saccharomyces castellii.
  • Designed sequence-specific small interfering RNAs (siRNAs) targeting plasmid-encoded selection markers.
  • Developed a chemically inducible system for regulating plasmid copy number.

Main Results:

  • Achieved up to a 7.13-fold amplification in plasmid copy number.
  • Demonstrated an 18.6-fold increase in lycopene titers by modulating carotenoid biosynthesis.
  • Established a novel RNAi-mediated gene dosage control platform.

Conclusions:

  • The developed RNAi-mediated system enables dynamic plasmid copy number regulation in S. cerevisiae.
  • This strategy significantly enhances production performance in microbial cell factories.
  • Offers new perspectives for metabolic engineering and synthetic biology applications.