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Related Concept Videos

The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

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Updated: Jun 22, 2026

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

DNA replication fork proteins.

Ulrich Hübscher1

  • 1Institute of Veterinary Biochemistry and Molecular Biology, University of Zürich-Irchel, Zürich, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)
|July 1, 2009
PubMed
Summary
This summary is machine-generated.

DNA replication proteins at the fork are crucial for genome integrity. They participate in DNA repair and damage sensing, ensuring proper cell function and replication.

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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • DNA replication involves numerous protein factors working in concert.
  • Replication factors are increasingly recognized for their roles beyond replication, in maintaining genome integrity.
  • These proteins are essential for various DNA transaction events.

Purpose of the Study:

  • To review the properties of DNA replication proteins functioning specifically at the replication fork.
  • To highlight the multifaceted roles of these proteins in genome maintenance.
  • To emphasize their involvement in DNA repair and checkpoint pathways.

Main Methods:

  • Literature review of studies on DNA replication proteins.
  • Analysis of the interplay between replication factors and DNA transaction events.
  • Summarization of findings on protein properties and functions.

Main Results:

  • DNA replication proteins are implicated in multiple DNA repair pathways, including base excision repair, nucleotide excision repair, double-strand break repair, and mismatch repair.
  • Several replication proteins are essential for sensing and transducing DNA damage signals through checkpoint cascade pathways.
  • These proteins contribute to both replication and protection of the genome.

Conclusions:

  • DNA replication fork proteins are integral to maintaining genome integrity.
  • Their functions extend to DNA repair and damage response, crucial for cellular function.
  • Understanding these proteins is key to comprehending genome stability and cell viability.