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Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells
Published on: October 13, 2015
Flow cytometric quantification of chlamydial infection in cell culture
Michael Grün1, Marita Liebisch, Helga Sauerwein
1Institute of Nutrition, Friedrich-Schiller-University of Jena, Germany. michael.gruen@uni-jena.de
Journal of Microbiological Methods
|July 1, 2009
Abstract:
A flow cytometric method was developed, which allows fast and efficient analysis of cell cultures infected with chlamydiae. The proportion of positive cells increased with the infectious dose and correlated with chlamydia copy numbers calculated from real-time PCR. While retaining the advantages of single-cell analysis, flow cytometry allows handling of large sample numbers and counterstaining for additional marker proteins.
