Related Experiment Video
Updated: Jun 22, 2026
Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
Published on: March 11, 2020
[Initial RAPD analysis of Isatis indigotica somatic clones' resistance to root rot disease]
Gui-Ping Chen1, Shao-Ying Ke, Yu-Qin Chen
1Department of Biology Science, Tangshan Teachers College, Tangshan 063000, China. chenguiping968@sohu.com
Objective:
To make initial identification on Isatis Indigotica Somatic Clones' resistant mutant.
Methods:
The resistant plants were selected under the treatment of root-rot disease crude toxin. The DNA of the same clones and their parents were analysed by RAPD technology.
Results:
We got initial resistance of the plants and selected 12 primers of which most were identical but the less were different. The genetic background of the somatic clones was identical but the individuals were mutated on the DNA level.
Conclusion:
It provides experimental foundation for further study on separating resistant mutant and resistant gene.
