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Related Experiment Video

Updated: Jun 22, 2026

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy
08:48

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

Published on: November 22, 2019

Developing compact multiphoton systems using femtosecond fiber lasers.

Shuo Tang, Jian Liu, Tatiana B Krasieva

    Journal of Biomedical Optics
    |July 2, 2009
    PubMed
    Summary
    This summary is machine-generated.

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    A compact femtosecond fiber laser at 1030-nm wavelength is demonstrated for multiphoton imaging. This technology shows promise for creating portable, all-fiber multiphoton systems and endoscopes.

    Area of Science:

    • Biomedical Optics
    • Laser Physics
    • Microscopy

    Background:

    • Multiphoton imaging offers deep tissue penetration and reduced phototoxicity.
    • Compact and portable laser sources are crucial for advancing in-vivo and point-of-care imaging applications.
    • Femtosecond fiber lasers provide high peak power and short pulse durations essential for nonlinear optical processes.

    Discussion:

    • A compact femtosecond fiber laser operating at 1030 nm was integrated into a multiphoton imaging setup.
    • The laser system utilizes a photonic bandgap fiber for output delivery.
    • Characterization of the laser includes pulse duration (150 fs), average power (200 mW), and repetition rate (40 MHz).

    Key Insights:

    • The 1030-nm femtosecond fiber laser successfully excited intrinsic second-harmonic generation (SHG) signals in biological tissues (rat tail tendon, human skin).

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  • Two-photon excited fluorescence (TPEF) was effectively detected in exogenously stained human skin samples.
  • The compact size (200 x 160 x 45 mm) and fiber delivery are advantageous for system miniaturization.

    • Outlook:

    • The developed laser technology holds significant potential for the creation of compact, all-fiber-based, portable multiphoton imaging systems.
    • Future applications may include development of novel endoscopic imaging devices.
    • Further research can explore optimized laser parameters for enhanced imaging contrast and resolution in various biological tissues.