Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 22, 2026

Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales
09:56

Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales

Published on: August 21, 2019

Three-dimensional surface profile intensity correction for spatially modulated imaging.

Sylvain Gioux1, Amaan Mazhar, David J Cuccia

  • 1Boston University, Department of Biomedical Engineering, Boston, Massachusetts 02215, USA.

Journal of Biomedical Optics
|July 2, 2009
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Commercialization Report: Modulim Inc.

Biophotonics discovery·2026
Same author

Lighting the path: a narrative review of non-molecular intraoperative lung imaging modalities.

Journal of thoracic disease·2026
Same author

Rapid Quantitative Imaging of Heterogeneous Tissue Hemoglobin Dynamics Using Spatial Frequency Domain Imaging.

Journal of vascular research·2026
Same author

Optical and acoustic scattering in cutaneous neurofibromas: Implications for early detection.

The Journal of investigative dermatology·2026
Same author

Two-step regression analysis approach to assess burn wound severity using spatial frequency domain imaging.

Journal of biomedical optics·2026
Same author

Short-wave infrared (SWIR) spectroscopy and imaging of biological tissues: a decade of advancements (2016-2025).

Journal of biomedical optics·2025

This study introduces a noncontact method using structured light to accurately measure tissue optical properties (absorption and scattering). This technique improves accuracy for complex shapes and varying surfaces, paving the way for surgical imaging.

Area of Science:

  • Biomedical Optics
  • Medical Imaging
  • Optical Physics

Background:

  • Quantitative optical properties of tissues are crucial for medical diagnostics and treatments.
  • Accurate measurement of absorption (μa) and reduced scattering (μs') coefficients is challenging, especially for non-flat surfaces.
  • Existing methods often require contact or are limited in handling complex geometries.

Purpose of the Study:

  • To develop and validate a noncontact profile correction technique for quantitative wide-field optical measurement of tissue absorption and reduced scattering coefficients.
  • To integrate phase-shifting profilometry and modulated imaging into a single instrument for simultaneous surface and optical property acquisition.
  • To assess the accuracy improvement offered by the profilometry correction for complex sample shapes and orientations.

More Related Videos

Determining 3D Flow Fields via Multi-camera Light Field Imaging
14:25

Determining 3D Flow Fields via Multi-camera Light Field Imaging

Published on: March 6, 2013

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
13:43

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

Related Experiment Videos

Last Updated: Jun 22, 2026

Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales
09:56

Automated 3D Optical Coherence Tomography to Elucidate Biofilm Morphogenesis Over Large Spatial Scales

Published on: August 21, 2019

Determining 3D Flow Fields via Multi-camera Light Field Imaging
14:25

Determining 3D Flow Fields via Multi-camera Light Field Imaging

Published on: March 6, 2013

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
13:43

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

Main Methods:

  • Developed a single instrument projecting structured light for both phase-shifting profilometry and modulated imaging.
  • Acquired 3D surface geometry and diffuse reflectance intensity of phantoms and biological samples.
  • Applied geometric correction based on the acquired surface profile to extract optical properties (μa, μs').

Main Results:

  • Demonstrated accurate extraction of optical properties for flat and complex-shaped silicone phantoms and human fingers.
  • Achieved significant improvement (2- to 10-fold) in accuracy for absorption and reduced scattering coefficients using profilometry correction.
  • Successfully detected changes in optical properties, such as vascular constriction in a human finger.
  • Validated the technique for height variations up to 3 cm and tilt angles up to 40 degrees.

Conclusions:

  • The noncontact profile correction technique significantly enhances the accuracy of quantitative optical property measurements.
  • The integrated instrument and method are suitable for complex sample geometries and varying surface orientations.
  • This approach provides a foundation for utilizing structured light in quantitative surgical imaging applications.